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Learning Center - Case Studies Protocols Case Studies

Protein Expression using Insect Cell

  • Baculovirus used to express recombinant protein in SF9and Hi.5 cells
  • System200 used to generate 100L of SF9 or Hi.5 cell
  • 100L batch is then aliquoted into 20 Cellbags10L, 2 bags per System20/50
  • Each Cellbag10L is used for specific protein vector
  • Result is 60 protein constructs expressed weekly with staff of three!

200L

20L

20L

20L

20L

20L

20L

20L

20L

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20L